Pepstatin pentapeptide ne, mai hana aspartyl protease inhibitor ta halitta, wanda zai iya hana aspartic protease da acid protease na daban-daban microorganisms.Pepstatin galibi yana ɓoye ne ta nau'in Streptomyces kuma Streptomyces ne ke samarwa.Yana iya hana pepsin, pepsin D da angiotensin-releasing enzyme, kuma yana da tasirin warkewa akan gyambon ciki, hawan jini na koda, arthritis, carrageenan edema da sauran cututtuka.
Pepstatin shine babban mai hana aspartyl proteases kamar pepsin, cathepsin D, da renin.Wannan pentapeptide na halitta wanda aka ware daga actinomycetes ya kasance tsawon shekaru masu yawa mai hana renin in vitro.Pepstatin bai keɓanta da renin ba kuma ba ya narkewa cikin ruwa.Abubuwan da suka samo asali na pepstatin sun ƙaru da ƙarfi da ƙayyadaddun renin ta umarni masu girma da yawa.Pepstatin ya ƙunshi γ amino acid statin wanda ba a saba gani ba wanda zai iya maye gurbin amino acid guda biyu a haɗin haɗin furotin kuma ya toshe raguwa saboda tsarin kwatancen. zuwa yanayin canji na peptide bond hydrolysis ta aspartyl proteases.
Lokacin da aka fitar da furotin daga ƙwayoyin da suka karye, ana iya fitar da proteases, waɗanda ke buƙatar hana su da sauri don kiyaye furotin daga lalacewa.A cikin tsarin fitar da furotin, ana buƙatar ƙara masu hana protease don hana proteolysis.Protease inhibitor a sarari yana nufin wani abu da ke ɗaure ga wasu ƙungiyoyi akan cibiyar aiki na ƙwayoyin protease, ta yadda aikin protease ya ragu ko ma ya ɓace, amma baya hana furotin enzyme.Hankalin nau'ikan proteases daban-daban zuwa furotin daban-daban ya bambanta, don haka ya zama dole don daidaita haɓakar ƙwayoyin cuta daban-daban.Saboda solubility na protease inhibitor a cikin ruwa yana da ƙasa sosai, ya kamata a ba da kulawa ta musamman ga mai hana protease ya kamata a haɗa shi sosai a cikin buffer don rage hazo na mai hana protease.Pepstantin A na iya hana ƙwayoyin ƙwayoyin acid kamar pepsin, angiotensin, collagenase, cathepsin D da chymosin.
Pepstatin A shine mai hana cathepsin d da e.Bayan da aka bi da kwayoyin HEK293 tare da nau'i daban-daban na pepstatin A na tsawon sa'o'i 24, an gano bayanin LC3Ⅱ da p62.Sakamakon ya nuna cewa pepstatin A na iya haɓaka maganganun LC3Ⅱ da P62 (P<0.05) ta hanyar dogaro da kashi.An yi amfani da 20μ g / ml pepstatin A don magance HEK293 a lokuta daban-daban, kuma an lura da tasirin lokuta daban-daban akan maganganun LC3II da p62.Sakamakon ya nuna cewa pepstatin A zai iya haɓaka-daidaita maganganun LC3II da p62 a cikin hanyar da ta dogara da lokaci.
Mu masu sana'a ne na polypeptide a kasar Sin, tare da shekaru masu yawa na kwarewa a cikin samar da polypeptide.Hangzhou Taijia Biotech Co., Ltd. ƙwararren ƙwararren masana'anta ne na polypeptide, wanda zai iya samar da dubun dubatan polypeptide albarkatun ƙasa kuma ana iya keɓance shi gwargwadon buƙatu.Ingancin samfuran polypeptide yana da kyau sosai, kuma tsabta zai iya kaiwa 98%, wanda masu amfani suka gane a duk faɗin duniya. Barka da tuntuɓar mu.
