GsMTx4 shine peptide 35-amino acid tare da haɗin disulfide guda huɗu waɗanda ke samar da maƙasudin kullin cysteine, wanda shine fasalin tsarin gama gari na yawancin peptides na gizo-gizo gizo-gizo wanda ke ba da kwanciyar hankali da ƙayyadaddun bayanai.Tsarin aikin GsMTx4 ba a cika cikakken bayani ba, amma an yi imanin cewa yana ɗaure zuwa ga ƙananan ƙwayoyin cuta ko transmembrane na MSCs na cationic kuma yana toshe buɗewar buɗaɗɗen su ko gating ta hanyar canza yanayin su ko tashin hankali.An nuna GsMTx4 don hana MSCs cationic da yawa tare da zaɓi daban-daban da ƙarfi.Misali, GsMTx4 yana hana TRPC1 tare da IC50 na 0.5 μM, TRPC6 tare da IC50 na 0.2 μM, Piezo1 tare da IC50 na 0.8 μM, Piezo2 tare da IC50 na 0.3 μM, amma ba shi da tasiri akan TR4PV10 ko sama da TR zuwa TR. μM.(Bae C et al 2011, Biochemistry)
An yi amfani da GsMTx4 azaman kayan aikin harhada magunguna don nazarin aiki da ka'idojin MSCs na cationic a cikin nau'ikan tantanin halitta da kyallen takarda.Wasu daga cikin misalan sune:
GsMTx4 na iya toshe MSCs waɗanda aka kunna ta hanyar shimfiɗawa a cikin astrocytes, ƙwayoyin zuciya, ƙwayoyin tsoka mai santsi da ƙwayoyin tsoka.Astrocytes sel ne masu siffar tauraro waɗanda ke tallafawa kwakwalwa da kashin baya.Kwayoyin zuciya su ne sel waɗanda ke yin tsokar zuciya.Kwayoyin tsoka masu laushi su ne sel waɗanda ke sarrafa motsin gabobin kamar ciki da tasoshin jini.Kwayoyin tsokar kwarangwal su ne sel waɗanda ke ba da damar motsi na son rai na jiki.Ta hanyar toshe MSCs a cikin waɗannan sel, GsMTx4 na iya canza kaddarorinsu na lantarki, matakan calcium, ƙanƙancewa da annashuwa, da kuma bayyanar cututtuka.Wadannan canje-canje na iya shafar yadda waɗannan kwayoyin ke aiki akai-akai ko a cikin yanayin cututtuka (Suchyna et al., Nature 2004; Bae et al., Biochemistry 2011; Ranade et al., Neuron 2015; Xiao et al., Nature Chemical Biology 2011)
GsMTx4 kuma na iya toshe wani nau'in MSC na musamman da ake kira TACAN, wanda ke da hannu cikin amsawar jin zafi.TACAN tashar ce da aka bayyana a cikin ƙwayoyin jijiya waɗanda ke jin zafi.Ana kunna TACAN ta hanyar motsa jiki, kamar matsa lamba ko pinching, kuma yana haifar da jin zafi.GsMTx4 na iya rage ayyukan TACAN kuma rage halayen ciwo a cikin nau'ikan dabbobi na ciwo na inji (Wetzel et al., Nature Neuroscience 2007; Eijkelkamp et al., Nature Communications 2013)
GsMTx4 na iya kare astrocytes daga gubar da kwayoyin halitta da ake kira lysophosphatidylcholine (LPC) ke haifar da su, wanda shine matsakaicin lipid wanda ke haifar da lalacewa ga kwakwalwa da kashin baya.LPC na iya kunna MSCs a cikin astrocytes kuma ya sa su ɗauki nauyin calcium mai yawa, wanda ke haifar da damuwa na oxidative da mutuwar kwayar halitta.GsMTx4 na iya hana LPC kunna MSCs a cikin astrocytes kuma ya kare su daga guba.GsMTx4 kuma zai iya rage lalacewar kwakwalwa da inganta aikin jijiya a cikin mice da aka yi musu allura tare da LPC (Gottlieb et al., Journal of Biological Chemistry 2008; Zhang et al., Journal of Neurochemistry 2019)
GsMTx4 na iya daidaita bambance-bambancen ƙwayar jijiyoyi ta hanyar toshe takamaiman nau'in MSC da ake kira Piezo1, wanda aka bayyana a cikin ƙwayoyin jijiyoyi.Kwayoyin jijiyoyi sune sel waɗanda zasu iya yin sabbin ƙwayoyin jijiya ko wasu nau'ikan ƙwayoyin kwakwalwa.Piezo1 tashoshi ne da ke kunna shi ta hanyar alamu na inji daga muhalli, kamar taurin kai ko matsa lamba, kuma yana tasiri yadda ƙwayoyin jijiyoyi ke yanke shawarar irin tantanin halitta zai zama.GsMTx4 na iya tsoma baki tare da ayyukan Piezo1 kuma ya canza bambance-bambancen ƙwayar jijiyoyi daga neurons zuwa astrocytes (Pathak et al., Journal of Cell Science 2014; Lou et al., Rahoton Cell 2016)
Mu masu sana'a ne na polypeptide a kasar Sin, tare da shekaru masu yawa na kwarewa a cikin samar da polypeptide.Hangzhou Taijia Biotech Co., Ltd. ƙwararren ƙwararren masana'anta ne na polypeptide, wanda zai iya samar da dubun dubatan polypeptide albarkatun ƙasa kuma ana iya keɓance shi gwargwadon buƙatu.Ingancin samfuran polypeptide yana da kyau sosai, kuma tsabta zai iya kaiwa 98%, wanda masu amfani suka gane a duk faɗin duniya. Barka da tuntuɓar mu.
